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a gDNA standard is provided which can be used to test the sensitivity of qPCR assays for gDNA background
enhanced stabilization of mRNA
Compatible with a variety of fluorogenic probe chemistries
variation between qPCR processing runs is observed due to different baseline subtractions and threshold settings
The Preamplification primer mix contains primers for all the GrandPerformance assay targets that will amplify them all in proportion for downstream singleplex qPCR analysis
HL-Exol Reactions:HL-ExoI 20-2500 a gDNA standard is providedHL ExoI is a 3 5 exonuclease, specific for single stranded DNA. HL ExoI is active at temperatures ? 25C, and inactivated by 1 min incubation at 80C, or 15 min at 60C
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